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KMID : 0613820060160030505
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Journal of Life Science
2006 Volume.16 No. 3 p.505 ~ p.509
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HQNO-sensitive NADH:DCIP Oxidoreductase of a Pathogenic Bacillus cereus Causing β-Hemolysis
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Kim Young-Jae
Park Ki-Tae
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Abstract
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Membranes prepared from Bacillus cereus KCTC 3674, grown aerobically on a complex medium, oxidized NADH exclusively, whereas deamino-NADH was little oxidized. The respiratory chain-linked NADH oxidase system exhibited an apparent Km value of about 65 μM for NADH. Interestingly, the activity of NADH:DCIP oxidoreductase on NADH oxidase system was decreased remarkably by Na? or K?, and its optimal pH was 5.5. The activity of NADH:DCIP oxidoreductase was very resistant to the respiratory chain inhibitors such as rotenone, capsaicin, and AgNO₃, whereas it was inhibited by about 40% with 40 μM 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO). From the results, we suggest the possibility that the aerobic respiratory chain-linked NADH oxidase system of B. cereus KCTC 3674 may possess the HQNO-sensitive NADH:DCIP oxidoreductase lacking an energy coupling site.
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KEYWORD
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Bacillus cereus KCTC 3674, aerobic respiratory chain, NADH:DCIP oxidoreductase
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